Weanling female Sprague Dawley rats were fed diets containing 22mg pyridoxine HCl/kg diet(control diet) and 1.2mg pyridoxine HCl/kg diet (deficient diet). They were fed thier diets throughout growth, gestation and lactation. After the pups were born and weaned, deficient group was divided into two group. One(DC) switched to control diet and the other(DD) continued the same deficient diet until 35 weeks of age.
Body weight of deficient group was slightly lower than the control. But brain weight was not affected by pyridoxine depletion. Brain DNA contents of control group were higher than the deficient and significant at 2nd weeks. Brain RNA contents of deficient group were slightly lower than the control, but the effect of pvridoxine deficiency on RNA was smaller than the effect of pyridoxine deficiency on DNA.
The distribution of fatty acids in cerebrosides corresponded in general pattern, so the sum of C-24 : o and C24 : , was the majority in all groups and there are more long chain fatty acids (C2_´-Ca) in a-hydroxv fatty acid (HFA) than in normal fatty acid(NFA). As the increase of age short chain fatty acids decreased sharply and the increase of long chain fatty acid appeared, and the degree of unsaturation was proportionate to the increase of age, specifically in NFA. The significant difference among groups did not appear, but long chain fatty acids of NFA decreased slightly in the DD group and the degree of unsaturation changed in NFA.
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